High Content Screening Drug Discovery

In most pharmaceutical and biotechnology companies there is a need to always improve the quality of lead candidates.

High content screening drug discovery.

Recent developments in high content screening hcs technologies make it an attractive alternative for anti angiogenic drug discovery. High content screening hcs has played a significant role in infectious disease research and drug discovery to date and can be a strong tool in the age of covid 19 and beyond. Hence high content screening is a type of phenotypic screen conducted in cells involving the analysis of whole cells or. Paper phenotypic drug discovery with high content screening target based or phenotypic screening.

Hcs integrates high throughput methodologies with automated multicolor fluorescence microscopy to collect quantitative morphological and molecular data from complex biological systems. Discovery of a novel rock2 inhibitor with anti migration effects via docking and high content drug screening 1 january 2016 molecular biosystems vol. Read more optimising phenotypic screening single cell analysis versus 3d multicell analysis by dr richard m. A decade ago there was talk that high throughput screening hts was a contributory factor in drug discovery s declining productivity and that it stifled creativity and innovation.

Drug discovery in its early history used to be conducted mainly in a target agnostic fashion focusing on qualitative readouts from a living system cells tissue or organisms and asking the question did this entity affect the phenotype. 9 high content screening identifies inhibitors of the nuclear translocation of atf6. This demand resulted in the use of cell based screening as a method of choice in drug discovery 1 2 high content screening hcs is multiplexed functional cell based screening 3 4 5 6 hcs can be used in all aspects of drug discovery as an engine for driving. These types of assays are often referred to as high content screening or high content analysis hcs hca.

High content screening hcs also known as high content analysis hca or cellomics is a method that is used in biological research and drug discovery to identify substances such as small molecules peptides or rnai that alter the phenotype of a cell in a desired manner. It can be employed in drug discovery projects to optimize compound series on their safety profile and provide a risk assessment tool towards candidate selection.